2016 Fiscal Year Final Research Report
Development of vascular regenerative therapy using new tissue engineering
| Project/Area Number |
26462113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Saga University |
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Principal Investigator |
Itoh Manabu 佐賀大学, 医学部, 助教 (50555084)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 血管外科 / 組織工学 / 再生医療 / 人工血管 |
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| Outline of Final Research Achievements |
We generated scaffold-free tubular tissues from multicellular spheroids (MCS) composed of endothelial cells , smooth muscle cells and fibroblasts using a new tissue engineering, which were successfully implanted to nude rat and large animal (immunosuppressed mini pig) in an acute study. When the tubular tissues were matured in a perfusion system during more than one month, we confirmed resistance of more than 1000mmHg in the burst. Moreover, a layer structure similar to blood vessel was confirmed upon histopathologic testing and extracellular matrix production (outer side) as well as partial CD34-positive vascular endothelial cells on the inner side were confirmed.
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| Free Research Field |
心臓血管外科
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