Optimized magnitude of cryosurgery facilitating anti‑tumor immunoreaction in a mouse model of Lewis lung cancer

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462137
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Teikyo University
• Principal Investigator: Masafumi Kawamura 帝京大学, 医学部, 教授 (70169770)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 肺癌 / 腫瘍免疫 / クライオセラピー / 凍結壊死融解療法

Outline of Final Research Achievements

Cryosurgery, consisted of 60 sec freezing with liquid nitrogen and 60 sec sawing cycle, was performed to one tumor of bilateral femoral tumors that originated from Lewis Lung Cancer cell. Histologically, there was a significant correlation between the number of cycles of cryosurgery and the area of nonviable tumor area. 75% of the nonviable tumor area was observed in the 2 cycle group, and the abscopal tumor growth rate was significantly slower than that in the other groups. In immunohistochmistry, both the CD4 and CD8 positive T cells infiltration was significantly increased and Ki - 67 positive tumor cells were significantly decreased in the 2 cycle group . There was a significant increase in IL-1β, IL-2, IL-6, IL-12, and TNF-α in the 2-cycle group as compared with the other groups. Our data suggest that infiltration of both CD4 positive and CD8 positive T cells into the abscopal tumor via the increased proinflammatory cytokines could suppress the tumor growth.

Free Research Field

呼吸器外科