Establishment of the stem cell transplantation therapy by MUSE for moyamoya disease

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Tohoku University
• Principal Investigator: Miki Fujimura 東北大学, 医学系研究科, 准教授 (00361098)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: moyamoya disease

Outline of Final Research Achievements

The objective of this study was to establish stem cell transplantation therapy using pluripotent MUSE cells for the patients with moyamoya disease. Mice and rats were subjected to chronic cerebral ischemia and then underwent indirect pial synangiosis, in a consistent situation of indirect bypass for moyamoya disease. Two-staged carotid artery occlusion reproducibly provided chronic hypoperfusion and the development of pial synangiosis by our methods. To obtain more similar condition to moyamoya disease, we alternatively generated genetically-engineered mice of RNF213, a susceptibility gene for moyamoya disease. Spontaneous development of moyamoya disease was not evident in these mice, while RNF213-deficient mice demonstrated decreased ratio of regulatory T cells after immunologic adjuvant administration. Regarding MUSE cell preparation, using MUSE cells were successfully sorted by anti- SSEA-3/CD105 antibodies from cultured mesenchymal stromal cells.

Free Research Field

脳神経外科学