Effect of microRNA -21 in cerebral ischemia.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Kobe University
• Principal Investigator: Mizukawa Katsu 神戸大学, 医学部附属病院, 非常勤講師 (80403260)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: cerebral infarction / microRNA / metabolome

Outline of Final Research Achievements

In the mouse brain of cerebral ischemia (120-min middle cerebral artery occlusion (MCAO)), microRNA 21 (miR-21) expression was significantly increased, although miR-21 was not increased in 30-min MCAO brain. next, we performed a comparative metabolic analysis of the effects of cerebral ischemia on the metabolism. Metabolic profiling by gas-chromatography/mass-spectrometry analysis showed clear separation between the ischemia and control group. citrate, GABA, Grycerol, and Glysine etc. were significantly increased in MCAO brain, on the other hand, Glutamate, Ribrose-5P, Fructose-6P, Pyrvate, Glucose, etc. were significantly decreased in MCAO brain. The decreases of fructose 6-phosphate and ribulose 5-phosphate suggested enhancement of the pentose phosphate pathway (PPP) during cerebral ischemia.

Free Research Field

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