Therapy for cerebral infarction using infiltrated cells in ischemic brain

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462162
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Ehime University
• Principal Investigator: Kumon Yoshiaki 愛媛大学, 医学系研究科, 寄附講座教授 (80127894)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脳梗塞 / マクロファージ

Outline of Final Research Achievements

Two types of macrophages in lesion core of rat stroke model were identified according to NG2 chondroitin sulfate proteoglycan (NG2) and CD200 expression. NG2+ macrophages were CD200-, and vice versa. Although CD200+ macrophages cannot be classified as either M1 or M2, CD200+ macrophages expressed two splice variants of CD200 that are CD200L and CD200S.
Rats transplanted with C6-CD200S cells in the brain survived for a longer period than those transplanted with original C6 or C6-CD200L cells. The C6-CD200S tumors were smaller than the C6-CD200L or C6-original tumors, and many apoptotic cells were found in the tumor cell aggregates. Tumor-associated macrophages in C6-CD200S tumors displayed dendritic cell -like morphology and CD86 expression. These results suggested that the inflammatory reaction induced by CD200S is superior to the contrary function by CD200L in the ischemic brain.

Free Research Field

脳神経外科学