2016 Fiscal Year Final Research Report
Genome-wide epigenetic analysis of glioma and glioma stem-like cells
Project/Area Number |
26462177
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurosurgery
|
Research Institution | Kyorin University |
Principal Investigator |
|
Research Collaborator |
AIHARA KOKI
OTANI RYOHEI
YANAGISAWA SHUNSUKE
OMATA MAYU
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 神経膠腫 / エピジェネティクス / メチル化 / 幹細胞 / 悪性転化 |
Outline of Final Research Achievements |
Methylation markers for glioma malignant progression identified by genome-wide methylation analysis were validated using MassARRAY. The result was highly correlated with Infinium methylation analysis. Many glioma samples, glioma cell line, and glioma stem cells were validated for the methylation markers. Methylation profile of glioma stem cells were similar to that of G-CIMP demethylated group, suggesting malignant progression with demethylation was related to glioma stemness. Demethylation sites in malignantly progressed samples were poorly enriched in Histon methylation marks such as H3K4me3 and H3K27me3 in embryonal stem cells.
|
Free Research Field |
脳神経外科学
|