Functional analysis of Methyl-CpG-binding domain protein 1 in differentiation process of malignant brain tumor

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Kochi University
• Principal Investigator: UEBA Tetsuya 高知大学, 教育研究部医療学系臨床医学部門, 教授 (00314203)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 膠芽腫 / エピジェネティクス

Outline of Final Research Achievements

MBD1 (Methyl-CpG-binding domain protein 1) binds to methylated CpG and is involved in transcriptional repression and then plays an essential role in transmitting epigenetic information. In glioblastoma (GBM), aberrant splicing of MBD1 has been observed as a post transcriptional mechanism. Shorter isoform of MBD1 is little expressed in normal cells, but is predominantly expressed in GBM. In this study, we analyzed the splicing regulation and function of the tumor-specific isoform. A reporter system was developed for detecting the aberrant splicing of MBD1. Knockdown of the tumor-specific isoform induced growth inhibition in GBM cells. These results suggest that the aberrant splicing of MBD1 contributes to oncogenesis and oncogenic phenotypes.

Free Research Field

脳神経外科学