Novel bone substitutes using Freeze-Dried Platelet-Rich Plasma for spinal fusion surgery.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462228
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: Orita Sumihisa 千葉大学, 大学院医学研究院, 助教 (60638310)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 多血小板血漿 / 新鮮凍結 / 骨癒合 / 組織修復

Outline of Final Research Achievements

Fresh platelet-rich plasma (PRP) accelerates bone union in rat model. However, fresh PRP has a short half-life. We suggested freeze-dried PRP (FD-PRP) prepared in advance and investigated its efficacy on bone union in vivo. Spinal posterolateral fusion (PLF) was performed on 8-week-old male Sprague-Dawley rats, showing that FD-PRP accelerated bone union comparable to fresh PRP and BMP (human recombinant bone morphogenetic protein 2) by remodeling the bone with thinner but more tangled trabecular bone with rigidity. Histologically, the trabecular bone had thinner and more branches in the FD-PRP. And the biomechanical strength was high comparable to autologous bone. In conclusion, FD-PRP provides bone union ability comparable to fresh PRP and BMP, and can be prepared in advance safely and at low cost.

Free Research Field

脊椎外科