The evaluation of protein expression and cyclic tensile strain of human OPLL

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462235
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Fukui
• Principal Investigator: Sugita Daisuke 福井大学, 学術研究院医学系部門, 助教 (90596678)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: OPLL / インディアンヘッジホッグ / 内軟骨骨化 / マイクロアレイ

Outline of Final Research Achievements

The genes related to chondrogenesis, including Ihh, Sox9, and Runx2, were upregulated after a 24-hour strain exposure. Furthemore, the protein expression levels of Gli2 and Gli3 were upregulated after a 6-hour cyclic tensile strain exposure, the protein expression levels of Ihh, Sox9, and Runx2 were upregulated after a 12-hour strain exposure. In addition, the Ihh, Sox9, Gli2, and Gli3 proteins were expressed in the proliferating chondrocyte layer, Runx2 was expressed in the hypertrophic chondrocyte layer, and PTHrP was expressed in both layers at the ossification front of OPLL. Our results suggest that cyclic tensile strain might enhance the expressions of some genes and proteins related to differentiation or maturation of chondrocytes, and Ihh and its related signaling might play a key role in human OPLL.

Free Research Field

脊椎後縦靭帯骨化症