Investigation of minimum invasive fusion surgery using injection of novel induced pluripotent stem cells cell

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462246
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka City University
• Principal Investigator: Suzuki Akinobu 大阪市立大学, 大学院医学研究科, 講師 (00445016)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: iPS細胞 / ヒト人工多能性幹細胞 / BMP遺伝子 / 骨形成タンパク / HSV-TK遺伝子 / 単純ヘルペスウイルス1型チミジンキナーゼ / 低侵襲脊椎固定術

Outline of Final Research Achievements

This study aimed to investigate the novel iPS cells, which can produce two types of protein; the bone morphogenetic protein-2 (BMP-2) to promote the bone fusion strongly, as well as the herpes simplex virus type 1- thymidine kinase (HSV-TK) to cause apoptotic cell death by ganciclovir administration. This novel iPS cell will allow spine surgeons to perform posterolateral lumbar spine fusion only with injection, instead of with current high invasive surgery. This study had started from 2014. During 2014-2016, we created the DNA vector to deliver the nucleic acids including the gene of BMP-2 and HSV-TK into nuclear of the target cells. Additionally, we confirmed the production of BMP-2 and HSV-TK from the myoblast cell of mouse, which was inrtoduced the genes. Hereafter, we are planning to introduce our vector to iPS cell, after the research to enhance the productivity of protein by the introduced cells.

Free Research Field

整形外科