Establishment of therapeutic strategy for osteoarthritis using introduction of mesenchymal stromal cells from subchondral bone.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: ADACHI Nobuo 広島大学, 医歯薬保健学研究院(医), 教授 (30294383)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 変形性関節症 / 骨穿孔術 / 間葉系幹細胞 / 軟骨損傷 / 軟骨修復

Outline of Final Research Achievements

The purpose of this study is to examine whether morphological differences between the knee and ankle joint affect the results of bone marrow stimulation technique for osteochondral defect in a rabbit model. Osteochondral defects were created at the knee and ankle. In the knee, osteochondral defects were created at the medial femoral condyle and patellar groove. At the ankle, defects were created in the talus at either a covered or uncovered area by the tibia. After that, drilling was performed. Histological evaluations were performed at 4, 8, and 12 weeks. The proliferation of chondrocytes and proteoglycan release of cartilage tissue were analyzed in vitro in both joints. At 12 weeks, hyaline cartilage with normal thickness was observed only for the defect at the covered area of the talus. No significant differences of proliferation and proteoglycan release were detected between the knee and ankle. These results suggest that the congruent joint is advantageous for osteochondral repair.

Free Research Field

整形外科学