Experimental study of bone reconstruction using osteogenic matrix cell sheet

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462276
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Nara Medical University
• Principal Investigator: omokawa shohei 奈良県立医科大学, 医学部, 教授 (70597103)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 細胞シート / 骨欠損 / 骨形成能

Outline of Final Research Achievements

We investigated usefulness of cryopreserved osteogenic matrix sheet injection to enhance bone formation in rat femur defect model.
Three groups of sheet injections (fresh, slowly/rapidly cryopreserved) into rat dorsum were compared in terms of cell viability, osteogenic potential. Cell viability in slowly-cryopreserved group decreased compared to fresh group. There was no significant difference regarding osteocalcin mRNA expressions 4 weeks after injection. The other three groups (sham as control, slowly/rapidly cryopreserved) injections into segmental bony defect were compared regarding histology and biomechanical property. Histological observations showed extensive bone formation in the cryopreservation groups. Maximum failure strength of femur in slowly-cryopreserved group demonstrated significantly higher than that in the sham group during 6 weeks after injection. Slowly cryopreserved/thawed osteogenic matrix sheet injection enhanced bone formation in rat femur bone defect model.

Free Research Field

整形外科、手の外科、再建外科