Roles of AMP-activated protein kinase in bone metabolism

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462289
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Gifu University
• Principal Investigator: TOKUDA HARUHIKO 岐阜大学, 大学院医学系研究科, 非常勤講師 (10397325)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨代謝 / エネルギー代謝 / 骨芽細胞 / AMPキナーゼ / プロスタグランジン / オステオプロテジェリン / MAPキナーゼ

Outline of Final Research Achievements

AMP-activated protein kinase (AMPK), a key enzyme sensing cellular energy metabolism, is currently known to regulate multiple metabolic pathways. Osteoprotegerin plays a pivotal role in the regulation of bone metabolism by inhibiting osteoclast activation. We have previously reported that the activation of the mitogen-activated protein (MAP) kinase superfamily including p38 MAP kinase, p44/p42 MAP kinase and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) are involved in the synthesis of osteoprotegerin stimulated by prostaglandin D2 (PGD2), PGE1 or PGE2 in osteoblast-like MC3T3-E1 cells. On the basis of these findings, we herein investigated the implication of AMPK in the osteoprotegerin synthesis induced by PGD2, PGE1 or PGE2 in these cells. Our results strongly suggest that AMPK acts as a positive regulator in the osteoprotegerin synthesis stimulated by PGD2, PGE1 or PGE2 in osteoblasts.

Free Research Field

整形外科学