2016 Fiscal Year Final Research Report
Epigenetic regulation of miRNA induced by mechanical stress in human chondrocytes
| Project/Area Number |
26462299
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | miRNA / 軟骨代謝 / メカニカルストレス / マイクロアレイ解析 / エピジェネティクス / HDAC / 疾患修飾性OA治療薬 |
|---|
| Outline of Final Research Achievements |
Human chondro-osteophytes were obtained at the time of total joint arthroplasty from knee joints and hip joints of patients with osteoarthritis (OA). ADAM12, one of the target gene of miRNA29-b was observed in chondrocytes of the proliferative and hypertrophic zones in human chondro-osteophytes by immunohistochemistry. ADAM12 mRNA was up-regulated during chondrogenic differentiation in ATDC5 cells, before up-regulation of type X collagen mRNA. In patients with OA, mechanical forces acting on the sites of soft tissue attachments at the joint margins play a primary role in chondro-osteophyte formation and may cause joint pain leading to impaired activity of daily living. In addition, it has been reported that the haplotypes in the ADAM12 gene are strongly related to the risk of clinical knee OA. Modulation of signaling pathway of miRNA29b-ADAM12 axis may contribute the future development of a novel therapy against OA.
|
| Free Research Field |
医歯薬学（外科系臨床医学・整形外科学）
|
