2016 Fiscal Year Final Research Report
Clarification of collagen degeneration mechanisms by NIR-Raman spectroscopy and development of a novel optical biopsy system
| Project/Area Number |
26462320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ヒト軟骨 / 細胞外マトリックス / 微細構造 / ラマン分光 |
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| Outline of Final Research Achievements |
Near-infrared (NIR) Raman microprobe spectroscopy was applied to non-invasive characterization of biochemical structure of extracellular matrix (ECM) in human articular cartilage, a step forward along the path of in-vivo diagnostic application of osteoarthritis (OA). OA severity was microscopically classified into five grades (Grade 0-VI) on cartilage surfaces, according to the Collins scale. NIR-Raman spectra were collected from the selected surface regions with different OA severity. A systematic increase in relative intensity ratio between the Raman bands located at 1241 and 1269 cm-1 (amide III ratio) was observed with increasing degradation grades, which could be associated with disordered collagen orientation (increased formation of random coil). In addition, we developed 3-mm diameter fiber Raman probe which can perform the in-situ characterization of local OA severity as optical biopsy system.
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| Free Research Field |
生体材料学、分光学
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