2016 Fiscal Year Final Research Report
T cell and macrophage derived proinflammatory cytokines-induced joint destruction
| Project/Area Number |
26462321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 関節リウマチ / 滑膜 / 炎症性サイトカイン |
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| Outline of Final Research Achievements |
In this study, mRNA expression levels of IFN-γ, IL-17 and TNF-α were investigated in individual patients with RA and the correlations between pairs of these three pro-inflammatory cytokines were analyzed. Synovial tissues were obtained during arthroplasties from 24 RA patients and qRT-PCR for IFN-γ, IL-17 and TNF-α was performed.
Based on relative expression levels of the three pro-inflammatory cytokines, patients were classified into 3 major types; an IFN-γ plus TNF-α-dominant type, an IL-17-dominant type, and the other type. TNF-α expression levels were correlated with IFN-γ. In addition, there was a negative correlation between TNF-α and IL-17, and IFN-γ and IL-17. Median relative expression levels of TNF-α have no significant difference between the TNFi and the csDMARDs groups. In the rheumatoid synovial tissues, expression levels of TNF-α were modulated in parallel with IFN-γ, and TNF-α and IL-17, or IFN-γ and IL-17 did not co-express at high levels.
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| Free Research Field |
整形外科学
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