Effect of anesthetics on human cancer cell proliferation under diabetic conditions

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Nara Medical University
• Principal Investigator: Kawaraguchi Yoshitaka 奈良県立医科大学, 医学部, 非常勤講師 (90433333)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 全身麻酔薬 / 高血糖 / 増殖能 / 活性酸素種 / ヒト癌細胞株

Outline of Final Research Achievements

We investigated the effect of volatile anesthetics, especially in conditions of high glucose and insulin, on cell proliferation in the human colon or hepatocellular cancer cell lines (HCT116, HT29, and HepG2). First, 1% sevoflurane for 6 h potentially enhanced cell proliferation via KATP channels in colon cancer cells. Second, 1% sevoflurane exposure enhanced cell proliferation in conditions of high glucose (300mg/dL), treated with 0.05 mg/l insulin in HepG2 cells. In addition, we found that 2% sevoflurane for 1 h leads to increased intracellular reactive oxygen species (ROS) concentrations and 200mg/dL of glucose for 3h reduces ROS concentrations potentially via increased osmotic pressure.

Free Research Field

麻酔科学