2016 Fiscal Year Final Research Report
A new endocrinological mechanism of septic shock and vitamin C supplementation in septic shock under real-time vitamin C monitoring with electron spin resonance spectroscopy
| Project/Area Number |
26462365
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology
|
|---|
| Research Institution | Oita University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
KOGA Hironori
ISHIGAMI Akihito
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 敗血症 / ビタミンC / 酸化ストレス / 下垂体後葉ホルモン / 副腎髄質ホルモン / 副腎皮質ホルモン / カテコラミン / コルチゾール |
|---|
| Outline of Final Research Achievements |
Senescence marker protein-30 (SMP30) / gluconolactonase (GNL) knockout (KO) mice were divided into vitamin C (VC) (+) or VC (-). VC (+) mice were given water containing 1.5 g/L ascorbic acid (AA), whereas VC (-) mice received water without AA. Mice were intraperitoneally injected with lethal dose of lipopolysaccharide (LPS) evaluated physiological parameters and liver and lung injury at 3h after LPS. These results show no significant differences both groups. We speculate that sacrifices after LPS may be too early.
Currently SMP30/ GNL KO mice are receiving water without AA and will become VC (-) mice in October of this year. In next study, high dose of AA or saline will be intraperitoneally administered to VC (-) mice 30 min before lethal dose of LPS injection. At 6-12h after LPS, pituitary-adrenal hormones, oxidative stress markers, inflammatory markers and various organ injuries are going to be measured and evaluated.
|
| Free Research Field |
麻酔、周術期管理
|
