2016 Fiscal Year Final Research Report
The efficacy of using microRNAs in platelets as a method of platelet function monitoring based on next-generation sequencing
Project/Area Number |
26462370
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Anesthesiology
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
Yoshinobu Nakayama 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90568198)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 血小板 / microRNA |
Outline of Final Research Achievements |
Currently, platelet function monitoring in the perioperative field has very limited clinical relevance. The present study was aimed to investigate the efficacy of using microRNAs in platelets as a method of platelet function monitoring. In the first step, we stimulated platelets with drugs and the application of shear stress, and compared the microRNA expressions in platelets between pre- and post-stimulation using next-generation sequencing technology. Comprehensive analyses by next-generation sequencing identified several microRNAs (including mir-155) exhibiting significant expression changes. Focusing on the microRNAs showing significant changes in the first step, we will perform the next step using antiplatelet agents.
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Free Research Field |
周術期学
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