The efficacy of using microRNAs in platelets as a method of platelet function monitoring based on next-generation sequencing

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462370
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Yoshinobu Nakayama 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90568198)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血小板 / microRNA

Outline of Final Research Achievements

Currently, platelet function monitoring in the perioperative field has very limited clinical relevance. The present study was aimed to investigate the efficacy of using microRNAs in platelets as a method of platelet function monitoring. In the first step, we stimulated platelets with drugs and the application of shear stress, and compared the microRNA expressions in platelets between pre- and post-stimulation using next-generation sequencing technology. Comprehensive analyses by next-generation sequencing identified several microRNAs (including mir-155) exhibiting significant expression changes.
Focusing on the microRNAs showing significant changes in the first step, we will perform the next step using antiplatelet agents.

Free Research Field

周術期学