The analysis of plastic change in spinal dorsal after the peripheral nerve injury by the high-speed image analytical method

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462378
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Niigata University
• Principal Investigator: BABA Hiroshi 新潟大学, 医歯学系, 教授 (00262436)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脊髄後角 / 糖尿病性神経障害性疼痛 / 細胞内Caイメージング / 可塑性変化 / 脊髄スライス / プレガバリン / 後根刺激 / ラット

Outline of Final Research Achievements

Pregabalin is thought to exert its therapeutic effect in neuropathic pain by inhibiting the activity of spinal dorsal horn(DH) neurons. However, whether a clinical concentration of pregabalin can cause acute inhibition of DH neurons in the spinal cord is controversial. To address this issue, we undertook intracellular Ca2+imaging studies using spinal cord slices, and examined if pregabalin acutely inhibits the primary afferent stimulation-evoked excitation of DH neurons in normal rats and in rats with painful diabetic neuropathy.Clinically relevant(10 μM) and a very high concentration of pregabalin(100 μM) did not affect dorsal roots timulation-evoked Ca2+ signals in normal and diabetic neuropathic rats.These results suggest that the activity of DH neurons in the spinal cord is not inhibited acutely by clinical doses of pregabalin. It is very unlikely that an acute inhibitory action in the DH is the main analgesic mechanism of pregabalin in neuropathic pain states.

Free Research Field

麻酔科学