Antitumor effect of liposome incorporating mycolic acid of Mycobacterium bovis bacillus Calmette-Guérin in mouse model.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462397
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: University of Tsukuba
• Principal Investigator: MIYAZAKI Jun 筑波大学, 医学医療系, 准教授 (10550246)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 膀胱癌 / ビーシージー / ミコール酸 / 癌免疫 / 感染免疫 / 脂質免疫

Outline of Final Research Achievements

Introduction and Objectives. Intravesical instillation of live bacillus Calmette-Guerin (BCG) is established immuno therapy of non-muscle invasive bladder cancer. Mycolic acids (MA) are the dominant lipids found in the cell wall of Mycobacterium species. We developed novel liposome incorporating MA and examined antitumor effects of MA. Methods. MA was isolated from M. bovis BCG Tokyo, and separated into three subclasses (alpha, methoxy and keto). Liposomes are prepared by sonication and extrusion. C57BL/6 mice were subcutaneously inoculated with a mixture of　MB49 cells and PBS or liposome with/without MA followed by additional injection of PBS or liposome. Tumor growth was monitored. Results. Tumor growth was significantly suppressed in mice treated with Keto MA-liposome (p=0.007 vs PBS). Conclusion. Keto MA-liposome showed antitumor effects in mouse.

Free Research Field

泌尿器科