Search for novel therapeutic target molecules focusing on cellular memory in iPS conversion of human kidney cancer cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: The University of Tokyo
• Principal Investigator: idei mana 東京大学, 医学部附属病院, 特任研究員 (00639213)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Toshiro 東京大学, 先端科学技術研究所, 特任教授 (10114125) ; ABURATANI Hiroyuki 東京大学, 先端科学技術研究所, 特任教授 (10202657)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: iPS細胞

Outline of Final Research Achievements

For the clones whose initialization was confirmed, the difference in DNA methylation was analyzed before and after iPS conversion, and a gene group defining universality and a gene group defining cancer were determined by comparison with human ES cell data . After fiscal year 27, iPS cells derived from human renal carcinoma established in fiscal year 26 were treated exosome purified from the cancer cell culture supernatant and induced to differentiate into cancer stem cells. Next, the induced cancer stem cells were transplanted into SCID mice, and tumors confirmed as cancer stem cells were excised using the proliferative ability as an index, and it was histologically confirmed that they are carcinomas, not teratomas. Finally, the effect on cancer by siRNA treatment or forced expression of the identified gene was examined. From the above, four novel therapeutic target genes have been identified.

Free Research Field

再生医療