2017 Fiscal Year Final Research Report
Establishment of order-made therapy by mediating cholesterol metabolism pathways in castration-resistant prostate cancer dells
| Project/Area Number |
26462432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Research Collaborator |
MURAMATSU HIROYUKI 愛知医科大学, 医学部, 助教 (60714847)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 去勢抵抗性前立腺癌 / 嫌気性解糖系 / 乳酸脱水素酵素 / アポトーシス / タキサン |
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| Outline of Final Research Achievements |
We investigated the role of LDH-A in mediating castration resistance of human prostate cancer (PC) cells by analyzing PC cell lines. LDH expression is strongly associated with docetaxel (DOC) sensitivity in PC cells. A specific LDH-A inhibitor sodium oxamate (SO) inhibited cell growth in PC cells, which was presumably caused by the inhibition of LDH-A protein expression. Synergistic cytotoxicity was observed after the combination therapy with DOC and SO in castration resistant LN-CSS cells, but not in parental LNCaP cells. The combination with DOC and SO caused cell cycle arrest in G2-M and SO promotes DOC-induced apoptosis in LN-CSS cells, which was partially caused by inhibition of DOC-induced increases in LDH-A expression. Our study may provide valuable information for the future development of targeted therapies for CRPC.
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| Free Research Field |
尿路悪性腫瘍
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