2016 Fiscal Year Final Research Report
The investigation into the novel smooth muscle contraction-relaxation mechanism of lower urinary tract and its clinical application
| Project/Area Number |
26462445
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 過活動膀胱 / 低活動膀胱 / 下部尿路症状 |
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| Outline of Final Research Achievements |
This study was aiming at three main targets. Firstly, the contribution of Exchange Protein directly Activated by cAMP (Epac) has been investigated to Ca sensitization of detrusor smooth muscle. In alpha-toxin treated skinned muscle fiber used human detrusor smooth muscle, Epac selective agonist, 8-pCPT-2'-O-Me-cAMP induced little relaxation in the contraction strip induced by 1 microM intracellular Ca. On the other hand, PKA selective cAMP, 6-Bnz-cAMP remarkably induced relaxation by approximately 50%. Taken together, cAMP-induced relaxation in human detrusor was mainly via PKA down stream but not via Epac system. Secondly, the troponin system had been tried to set up in smooth muscle. However, this project had not been successful due to the difficulty of designing transgenic mouse. The final project was to reveal the extracellular Ca and temperature dependency of contraction of urethral smooth muscle. The maximum tension force was obtained at 5mM [Ca]out and 37 celsius degree.
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| Free Research Field |
排尿機能生理学
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