New treatment strategy with mesenchymal stem cells for obstructive uropathy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462452
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Keio University
• Principal Investigator: Asanuma Hiroshi 慶應義塾大学, 医学部(信濃町), 准教授 (70245570)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 間葉系幹細胞 / 閉塞性腎症 / 閉塞性膀胱障害 / 上皮・間葉系形質転換 / 腎線維化 / 増殖因子 / サイトカイン / 細胞療法

Outline of Final Research Achievements

The effect of mesenchymal stem cells (MSCs) therapy on chronic obstruction-induced renal fibrosis has not previously been evaluated. Rats with partial ureteral obstruction underwent injection of fluorescent-labeled human and rat MSCs. From 3 days to 4 weeks later, the kidneys were harvested and the renal cortex analyzed for evidence of stem cell infiltration, epithelial-mesenchymal transition (EMT), renal fibrosis, and cytokine and growth factor activity.
MSCs were detected in the interstitium of the kidney more than 4 weeks post-obstruction. MSCs provided protection against obstruction-induced EMT and chronic renal fibrosis. While the mechanism of MSCs-induced renal protection during obstruction remains unclear, exogenous MSCs reduced obstruction-induced TNF-alpha levels, but did not alter TGF-beta1, VEGF, IL-10, FGF, HGF expression.

Free Research Field

医歯薬学・泌尿器科学