2016 Fiscal Year Final Research Report
Analysis of spermatogenesis with vacuolar structure under reduced genome quality in DNA repair deficient mice
| Project/Area Number |
26462460
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | DNA修復欠損マウス / 精子形成 / 精巣変性 / ゲノム不安定性 / 空胞様構造 |
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| Outline of Final Research Achievements |
We have showed that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] provide a valuable model for impaired spermatogenesis as well as for UV-induced skin tumors in XPA patients. Immunohistochemical and biochemical analysis using specific antibody of vacuolar formation was performed to clarify the pathology of vacuolar degeneration of Xpa mice testis. In addition, we created and observed monitoring mice of Xpa for vacuolar degeneration. We try to find therapeutic agents for impaired spermatogenesis by use of these mice. Through this analysis, we believe that the pathology of Xpa mouse testis and spermatogenesis with sperm head vacuoles under reduced genome quality will be elucidated and that there is a possibility of development of therapeutic drugs.
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| Free Research Field |
解剖学
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