Identification of molecules involved in the development and progression of uterine leiomyomas

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462492
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Yamaguchi University
• Principal Investigator: ASADA Hiromi 山口大学, 医学部附属病院, 講師 (90526906)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 子宮筋腫 / DNAメチル化 / 異種移植

Outline of Final Research Achievements

In this study, we aimed to establish the model to make the uterine leiomyomas in vivo and to identify molecules involved in the development and progression of uterine leiomyomas. We extracted 2 genes as candidate master gene by DNA methylome analysis and transcriptome analysis. We obtained a cell line in which the gene expression of SATB2 and NRG1 was altered and a cell line in which the expression of both genes was simultaneously altered. Comprehensive gene expression in these cell lines was examined by microarray, and a group of genes whose expression was changed in each cell line was extracted. These gene groups contained a plurality of genes whose expression was changed specifically in the uterine leiomyomas as compared with the normal myometrium. Currently, we are investigating to make the uterine leiomyomas in vivo by xenografts using these cell lines.

Free Research Field

生殖内分泌