2016 Fiscal Year Final Research Report
Estimation of second lymph node metastasis risk by evaluating of metastatic volume in sentinel node
Project/Area Number |
26462510
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Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Obstetrics and gynecology
|
Research Institution | Tohoku University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 子宮内膜癌 / 微小転移 / OSNA法 / RT-LAMP法 / センチネルリンパ節 / ITC / CK19 |
Outline of Final Research Achievements |
Ultrastaging is important for detecting the micrometastasis or isolated tumor cells in sentinel lymph node of endometrial cancer. Ultrastaging by step sectioning is burdensome and time comsuming. The present research clarified that a one-step nucleic acid amplification (OSNA) assay using cytokeratin 19 (CK19) mRNA was applicable for detecting lymph node metastases in endometrial cancer. In sentinel lymph node in endometrial cancer, 250 copies of CK19 mRNA by OSNA assay was an optimal cutoff value.
|
Free Research Field |
婦人科腫瘍学
|