2016 Fiscal Year Final Research Report
Establishment of a new tumor marker for trophoblastic neoplasia based on specific sugar chains of hCG
| Project/Area Number |
26462520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
Yamamoto Eiko 名古屋大学, 医学系研究科, 准教授 (10432262)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 絨毛性腫瘍 / 糖鎖 / ｈCG |
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| Outline of Final Research Achievements |
Choriocarcinoma cell line with GnT-IVa overexpression (Jar/GnT-IVa) was established and shRNA vectors were made for knockdown of GnT-III expression. GnT-III shRNA vectors were introduced into Jar/GnT-IVa. The levels of sugar chains were not changed by knockdown of GnT-III. Sandwich ELISA was performed using conditioned medium of Jar and GnT-IVa KD (Jar with knockdown of GnT-IVa expression), GSL-II lectin and a mouse antibody.
Whole cell lysate and conditioned medium were collected from Jar/GnT-IVa and control cells. Nano-LC/MS/MS analysis using the proteins showed that LAMP-2 was a target molecule of GnT-IVa.
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| Free Research Field |
絨毛性疾患
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