Roles of growth factors and oncogenes on cervical carcinogenesis in transgenic mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462528
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Ehime University
• Principal Investigator: Takashi Matsumoto 愛媛大学, 医学系研究科, 講師 (20346670)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 腫瘍発生学 / 子宮頸癌 / 動物モデル / トランスジェニックマウス

Outline of Final Research Achievements

In this study, we examined the impact of elevated several growth factors/oncogenes in cervical epithelium of transgenic mice. We generated transgenic mice those overexpressed transforming growth factor-a (TGF-α), c-src or insulin-like growth factor-1 (IGF-1) in epithelial basal cells driven by the keratin 14 (K14) or keratin 5 (K5) promoter. Histopathologic changes were assessed in uterine cervix of 12-month-old TGF-α transgenic mice, Src transgenic mice and IGF-1 transgenic mice, compared to age-matched non-transgenic littermates. The incidence of cervical intraepithelial neoplasia 3 (CIN3) was 37.5% (6/16), 30% (6/20) and 20.8% (5/24) in TGF-α transgenic mice, src transgenic mice and IGF-1 transgenic mice, respectively. Constitutive expression of growth factors/oncogenes, including TGF-α, c-src and IGF-1, and activation of the epidermal growth factor receptor in epithelium of uterine cervix may play an important role in tumorigenesis in this tissue.

Free Research Field

婦人科腫瘍学