Molecular mechanism for the onset of cervical cancer by analysis of HPV integration and DNA methylation

2017 Fiscal Year Final Research Report

• Project/Area Number: 26462539
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Suzuki Nao 聖マリアンナ医科大学, 医学部, 教授 (90246356)
• Co-Investigator(Kenkyū-buntansha): 上川 篤志 聖マリアンナ医科大学, 医学部, 研究技術員 (60534253)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 子宮頸がん / ヒトパピローマウイルス / ウイルス組込み

Outline of Final Research Achievements

Viral integration into the human genome upon infection is an important risk factor for various human malignancies. Human papilloma virus (HPV) integration is a key genetic event in cervical carcinogenesis. We performed a next-generation sequencing-based method for sequencing the HPV DNA integration sites, by high-throughput viral integration detection. We identified 12 HPV integration breakpoints in five cervical cancer cell lines and found variable levels of DNA methylation within the integrated HPV genomes. Our study accurately evaluated HPV integration sites in cervical cancer cells using high-throughput viral integration detection method providing basic evidence for HPV integration-driven cervical carcinogenesis.

Free Research Field

婦人科腫瘍学