2016 Fiscal Year Final Research Report
Preventing mucous metaplasia in chronic otitis media by inhibition of Math1
| Project/Area Number |
26462562
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nagoya City University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Math1 / TNF-α / レチノイン酸 / 杯細胞化生 |
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| Outline of Final Research Achievements |
A key issue in otitis media (OM) is mucous cell metaplasia in the middle ear mucosa, a condition for hyperproduction of mucus in the middle ear mucosa and development of chronic OM. We demonstrated that Math1 significantly increased the mucus-like cell numbers in the middle ear mucosa of mice. Math1, in the presence of TNF-a and retinoic acid (RA), synergistically promoted the differentiation of mouse middle ear epithelial cells (mMEEC) into mucus-like cells via upregulation of mucins and their chaperones:trefoil factors (TFFs) in vitro. We also demonstrated that Math1 activation (translocation to nucleus) was inhibited by epidermal growth factor receptor tyrosine kinase inhibitor, AG1478. In addition, mucous cell metaplasia induced by TNFα and RA was abrogated by AG1478. These results suggested that regulation of Math1 could prevent mucous cell metaplasia in chronic otitis media.
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| Free Research Field |
耳鼻咽喉科
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