Research of the inhibitory factors by regulation of epithelial cell polarity and organogenesis signaling in pharyngeal cancer invasion/metastasis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462613
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Sapporo Medical University
• Principal Investigator: KONDO ATSUSHI 札幌医科大学, 医学部, 講師 (40457718)
• Co-Investigator(Renkei-kenkyūsha): KOJIMA TAKASHI 札幌医科大学, 医学部, 教授 (30260764)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 咽頭がん / 細胞極性 / YAP / LSR / PAR3 / ASPP2 / HDAC

Outline of Final Research Achievements

The first, in pharyngeal squamous cell carcinomas (PSCC), a hippo pathway key molecule YAP which contributed to organogenesis signaling, was positive in the nuclei of all cancer cells. The changes in expression and localization of LSR (lipolysis-stimulated lipoprotein receptor) which was regulated by YAP were observed during the malignancy. In LSR-knockdown cancer cell line, upregulation of cell invasion and migration was observed. The next, epithelial cell polarity molecules PAR3 and the regulator ASPP2 (apoptosis stimulating proteins of p53-2) were upregulated in PSCC than dysplasia. Inhibitors of HDACs (histone deacetylases) which upregulated in PSCC, induced expression of PAR3 and ASPP2 and inhibited cancer cell invasion and migration. In conclusion, the regulation of epithelial cell polarity molecules LSR, PAR3 and ASPP2 and organogenesis signaling YAP may be an important to prevent invasion/metastasis of PSCC.

Free Research Field

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