2017 Fiscal Year Final Research Report
Analysis of oxidative stress in deep part of the glaucomatous eyes in mice by in vivo imaging using two photon excitation microscope
Project/Area Number |
26462660
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
|
Research Institution | Osaka University |
Principal Investigator |
USUI SHINICHI 大阪大学, 医学系研究科, 講師 (20546882)
|
Co-Investigator(Kenkyū-buntansha) |
松下 賢治 大阪大学, 医学系研究科, 講師 (40437405)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Keywords | 2光子励起顕微鏡 / 緑内障 / 酸化ストレス |
Outline of Final Research Achievements |
We established an observation system in vivo for analyzing oxidative stress in mouse glaucoma model. For the analysis, the special fixed base for observing the deep part of the eyeball in mice under inhalation anesthesia using a two-photon-induced microscope was prepared. We also examined a method of exposing the optic nerve. Furthermore, an intravenous route of the tail vein was secured, and the drug could be administered at any time. The dosage and time course of the drug required to observe the target tissue in vivo using this system was examined in wild type mice.
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Free Research Field |
緑内障
|