Elucidation of intraocular clearance system by lipid-degrading enzyme

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462665
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Sapporo Medical University
• Principal Investigator: HIRAOKA MIKI 札幌医科大学, 医学部, 講師 (80246983)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 房水浄化機構 / 眼内炎症 / リン脂質 / リン脂質分解酵素 / 自己免疫性ぶどう膜炎 / 酸化リン脂質

Outline of Final Research Achievements

The goal of this project is to clarify the involvement of phospholipids metabolism in ocular disease focusing the lysosomal phospholipase A2 (LPLA2).
In experimental autoimmune uveitis (EAU) model rats, the LPLA2 activity and the amount of phospholipids of the aqueous humor were concomitant with the extent of inflammation. The expression of LPLA2 and the production of oxidized phospholipid were observed in infiltrated macrophages. The LPLA2 deficient mice showed prolonged inflammation in endotoxin-induced uveitis. The clinical samples of aqueous humor from open-angle glaucoma patients showed higher LPLA2 activity than control group. In vitro, the hydrolysis of some truncated oxidized phospholipid (ox-PL) by LPLA2 was efficient compared to the non-oxidized phospholipids. Additionally, LPLA2 overexpressed cells catabolized truncated ox-PL and were protected from cell growth inhibition by ox-PL.

Free Research Field

眼の生化学・分子生物学