The effect of hydrogen for retinal oxidative stress

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462670
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nippon Medical School
• Principal Investigator: Takahashi Hiroshi 日本医科大学, 大学院医学研究科, 大学院教授 (00188046)
• Co-Investigator(Renkei-kenkyūsha): Ohsawa Ikuroh 独)東京都健康長寿医療センター, 研究副部長 (30343586)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 酸化ストレス / 白内障手術 / 超音波 / 水素 / 角膜内皮

Outline of Final Research Achievements

In phacoemulsification, ultrasound induces hydroxyl radical (・OH) formation, damaging corneal endothelium. The effects of H2 in phacoemulsification were evaluated in rabbits, comparing H2-dissolved and control solutions. The whole cornea was excised and subjected to image analysis for corneal edema, real-time semiquantitative PCR (qPCR) and immunohistochemistry for oxidative stress marker. Corneal edema was significantly less and the increases in anti-oxidative mRNA expressions were significantly suppressed in the H2 group. In addition, corneal endothelial cell expressions of oxidative stress markers were significantly lower in the H2 group. In conclusion, H2 dissolved in the ocular irrigating solution protected corneal endothelial cells from phacoemulsification-induced oxidative stress and damage.

Free Research Field

眼科