2016 Fiscal Year Final Research Report
Bone Marrow Mesenchymal Cell Transplantation for thin Corneal Stroma
| Project/Area Number |
26462690
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ケラトサイト / バイオイメージング / 骨髄線維芽細胞 / 再生医療 / 細胞増殖 |
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| Outline of Final Research Achievements |
Bone Marrow Mesenchymal Cell Transplantation for thin Corneal Stroma
ROSA26 (CAG-loxP-NeoR-loxP- mVenus -hGem (1/110) IRES2 mCherry -hCdt1 (30/120) ) knock-in mouse lines were newly developed by gene targeting technique using C57BL/6-derived ES cells for tissue specific cell cycle bio-imaging. These mice were mated with Keratocan-IRES2-nls-Cre to get bone marrow mesenchymal cell culture (BMC) . The BMCs were injected into artificial thin cornea stroma. The transplanted BMC proliferation were observed a confocal microscope or a multi-photon microscope in-vivo or ex-vivo. Corneal stromal opacities were appeared two weeks after BMC injection. Over-proliferation of BMCs increase the thickness of corneal stroma, but ruin its clarity.
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| Free Research Field |
眼科学
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