Development of novel treatment by using bacteriophage for ocular diseases induced by bacterial infection

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462692
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kochi University
• Principal Investigator: FUKUDA Ken 高知大学, 教育研究部医療学系臨床医学部門, 准教授 (70335751)
• Co-Investigator(Kenkyū-buntansha): 松崎 茂展 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (00190439) ; 内山 淳平 麻布大学, 獣医学部, 講師 (20574619)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 眼感染症 / 細菌 / バクテリオファージ / 角膜潰瘍 / 眼内炎

Outline of Final Research Achievements

We examined the therapeutic effects of bacteriophage eye drop on methicillin-resistant Staphylococcus aureus keratitis in rabbit. The clinical corneal score was significantly lower in rabbits treated by bacteriophage eye drop than in those treated by anti-biotic eye drop or control. The bacterial load in cornea among the vehicle-treated rabbit, anti-biotic-treated rabbit, and bacteriophage-treated rabbit were not significantly changed.
We then examined the bacterial endophthalmitis in rabbit and mouse. The injection Pseudomonas aeruginosa or Enterococcus faecalis into vitreous cavity in mice stably induced severe endophthalmitis including massive infiltration of neutrophils and destruction of tissue. Injection of phage into vitreous cavity in mice with endophthalmitis significantly suppressed clinical score and viable bacteria in the eyeball.

Free Research Field

眼科学