2016 Fiscal Year Final Research Report
Capability of vitreous fluid to enhance TGF-beta-induced Foxp3+ regulatory T cell conversion
| Project/Area Number |
26462696
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 眼免疫 |
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| Outline of Final Research Achievements |
In the present study, we determined whether vitreous fluid can promote the generation of Foxp3+ CD4+ T regulatory (Treg) cells in vitro. Vitreous fluid was collected from fresh porcine eyes, sterilized by filtration. Vitreous was used for culture at a final concentration of 25%. CD4+ T cells purified from pooled splenocytes from naive C57Bl/6J were stimulated with plate-bound anti-CD3 and anti-CD28 antibodies for 96h in the presence or absence of vitreous fluid. CD4+ T cells were analyzed for Foxp3 expression using flow cytometry. In some experiments, CD4+ T cells were cultured in the presence of vitreous fluid with or without TGF-beta1 or 2. Vitreous fluid alone did not increase the frequency of Foxp3+ Tregs. Although TGF-beta 1 or 2 induced Foxp3+ Tregs in vitro, the frequency of TGF-beta1/2 induced Foxp3+ Tregs increased significantly in the presence of vitreous fluid. These findings suggest that vitreous fluid may enhance TGF-beta-induced Foxp3+ Treg conversion.
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| Free Research Field |
ぶどう膜炎
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