2016 Fiscal Year Final Research Report
Role of microRNA in intraocular inflammation
| Project/Area Number |
26462697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ぶどう膜炎 |
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| Outline of Final Research Achievements |
Our recent study showed that the expression of Micro-RNA (miRNA) -223, 142, 21, and 146 in retina was elevated, whereas the expression of miRNA-181, 183, 124, and 331 was reduced during development of experimental autoimmune uveoretitnitis (EAU). The present study demonstrated increased expression of miRNA-223 and 146 in inner nuclear layer and inflammatory cells infiltrating in into retina using In Situ Hybridization. Furthermore, we investigated the changes of miRNA expression of iris/ciliary body of endotoxin induced uveitis (EIU) in rats. The number of cells infiltrating into aqueous humor peaked at 12 hours after injection of lipopolysaccharide (LPS). Microarray analysis demonstrated that the expression of miRNA-146, 155, 21, and 221 was increased at 12 hours, whereas the expression of miRNA-499,124, and 539 was reduced at 12 hours. These findings suggest that these up-regulated or down-regulated miRNAs may play roles for development and regulation of EIU as well as EAU.
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| Free Research Field |
眼炎症
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