2016 Fiscal Year Final Research Report
Network formation of enteric ganglion cells with Hirschsprung disease model
| Project/Area Number |
26462714
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Fujimura Takumi 慶應義塾大学, 医学部, 助教 (80573443)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ヒルシュスプルング病 / 神経堤 / 幹細胞 / 腸管 / モデルマウス / 蛍光タンパク質 / 移植治療 |
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| Outline of Final Research Achievements |
Hirschsprung disease is one of the major intestinal motility disorder, caused by the partial loss of intestinal ganglion cells derived from the absence or impaired migration of neural crest derived cells in early embryonic period. In this study, we established a new animal model for intestinal motility dysfunction by using the transgenic mouse strain, whose neural crest derived cells are labeled with green fluorescent protein. The analysis of the differentiation status of neural crest-derived cells and the functional abnormality of the gastrointestinal motility indicated that this model mouse continuously showed the similar phenotypes and pathophysiological courses of the human Hirschsprung disease patient. By creating a new disease model animal, it was possible to reveal a part of complicated mechanisms for the normal network formation and also a part of pathogenesis for intestinal motility disorder in vivo.
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| Free Research Field |
神経発生生物学
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