2016 Fiscal Year Final Research Report
Development of collagen binding VEGF-C for lymphangiogenesis
| Project/Area Number |
26462731
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | リンパ管新生 / リンパ浮腫 |
|---|
| Outline of Final Research Achievements |
We tried to make collagen binding VEGF-C and to verify its effect for developing a new treatment for lymphedema. A fusion protein of the collagen binding domain which is part of the gas gangrene toxin and the lymphangiogenic factor VEGF - C was designed and a fusion protein production system using insect cells was established. Collagen binding ability was confirmed by collagen adhesion test. However, proliferative activity against lymphatic endothelial cells could not be confirmed and phosphorylation signals could not be detected by MAPK signaling test. It is considered that there is a possibility that it has no physiological activity because it is not following the secretory pathway as a cause. We tried to design and produce a protein with signal peptide, but it did not lead to production.
|
| Free Research Field |
形成外科
|
