Analysis of ARDS regulation by autophagy and development of its treatment by microRNA.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26462757
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Kumamoto University
• Principal Investigator: Tashiro Takahiro 熊本大学, 医学部附属病院, 非常勤診療医師 (00613340)
• Co-Investigator(Kenkyū-buntansha): 木下 順弘 熊本大学, 大学院生命科学研究部(医), 教授 (30195341) ; 蒲原 英伸 熊本大学, 大学院生命科学研究部(医), 准教授 (90398222) ; 鷺島 克之 熊本大学, 医学部附属病院, 助教 (40336235) ; 廣佐古 進 熊本大学, 医学部附属病院, 非常勤診療医師 (70432995) ; 新森 大佑 熊本大学, 医学部附属病院, 診療助手 (70635789)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ARDS / Autophagy / microRNA / Exosome / Inflammation

Outline of Final Research Achievements

Homeostasis is maintained by autophagy due to decomposition of cellular organelles under biological stress. Alveolar-derived cell line (A549) and monocyte-derived cell line (THP-1) were used to elucidate the defense mechanism by alveolar cells and infiltrating leukocytes autophagy due to excessive inflammation of ARDS. Cells were stimulated with TNFalpha, Il-1 beta, LPS and the expression of protein LC3-II in antophagy induction was confirmed. This reaction was suppressed by the autophagy inhibitor. MicroRNAs were also profiled using serum from ARDS patients and microRNAs attributed to ARDS and Autophagy were identified.

Free Research Field

Critical Care Medicine