Elucidation of construction mechanism of extracellular environment around tooth germs via proteoglycan

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462777
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: Ida Hiroko 新潟大学, 医歯学系, 准教授 (60293213)
• Co-Investigator(Renkei-kenkyūsha): OHSHIMA Hayato 新潟大学, 医歯学系, 教授 (70251824)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: プロテオグリカン / コンドロイチン硫酸 / 歯胚 / 細胞外環境 / 顎顔面発育

Outline of Final Research Achievements

Chondroitin sulfate proteoglycan (CSPG) is one of major extracellular matrices and is known to play an important part in organogenesis. To elucidate the role of CS in tooth and craniofacial development and regeneration, we analyzed the craniofacial morphology in chondroitin sulfate synthetic enzyme knockout (CSKO) mice. In normal odontogenesis, the CS chain localized in immature dental papilla and periodontal ligament. In the differentiated dental pulp, CS chain restricted to the apex of tooth root. In CSKO mice, some of KO mice exhibited severe facial developmental defect. Although most of CSKO mice showed normal postnatal development, they exhibited the deformation of cranial bone and malocculusion. These results suggest that CS chain is necessary for normal tooth and craniofacial morphogenesis.

Free Research Field

口腔解剖学