Transcriptional regulation of TRPS1 in articular chondrocytes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462781
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Osaka University
• Principal Investigator: Abe Makoto 大阪大学, 歯学研究科, 講師 (40448105)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: エンハンサー / トランスジェニックマウス / TRPS1 / 転写因子 / 遺伝性疾患 / 関節軟骨 / 変形性関節症

Outline of Final Research Achievements

In order to identify the possible enhancer of TRPS1 gene, we generated a transgenic mice line those drive Cre recombinase activity under approximately 4kb proximal promoter sequence of the murine Trps1 gene (Trps1-Cre). We crossed our Trps1-Cre line with Cre reporter mice strain and detected the Cre activity from embryonic to postnatal stages. The Cre activity was observed in the organs such as central nervous system, articular chondrocytes, heart, and hair follicle. These regions are known to express Trps1 mRNAs. Cre activity detected in the cardiac region was somewhat unexpected since there were almost no reports regarding to involvement of Trps1 in cardiac development. We were able to detect Trps1 mRNA expression in the cardiac cushion.

Free Research Field

解剖学