The role of C-type lectin receptors in invasion of periodontal pathogenic bacteria into host cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462795
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Ohu University
• Principal Investigator: Tamai Riyoko 奥羽大学, 歯学部, 准教授 (90367566)
• Co-Investigator(Renkei-kenkyūsha): KIYOURA Yusuke 奥羽大学, 歯学部, 教授 (90194951)
• Research Collaborator: KOBAYASHI Michiyo
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯周病原性細菌 / Porphyromonas gingivalis / 侵入 / Ｃ型レクチン受容体 / galectin-1 / galectin-3 / galectin-4 / galectin-7

Outline of Final Research Achievements

Galectin-1 and galectin-3, which are C-type lectin receptors, bind to lipopolysaccharide (LPS) and prevent LPS-induced cytokine production by mouse splenocytes. In this study, we investigated the effects of galectin-1 and galectin-3 on adhesion to and invasion of the human gingival epithelial cell line Ca9-22 by Porphyromonas gingivalis. Recombinant galectin-1, but not galectin-3, up-regulated P. gingivalis adhesion and invasion, although both galectins bound similarly to P. gingivalis. Flow cytometry also revealed that Ca9-22 cells express low levels of galectin-1 and moderate levels of galectin-3. Ca9-22 cells in which galectin-3 was knocked-down did not exhibit enhanced P. gingivalis invasion. These results suggest that galectin-1 may exacerbate infectious disease by enhancing the invasion of host cells by anaerobic bacteria, while galectin-3 protects host cells from infection.

Free Research Field

口腔微生物学・免疫学