Early lymphatic vasculogenesis in the craniofacial region of embryonic mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462799
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: SATO KAORI 日本歯科大学, 生命歯学部, 講師 (90287772)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯学 / 病理学 / マウス胎仔 / 顎顔面発生 / リンパ管新生 / リンパ管内皮細胞 / 細胞分化制御 / 遺伝子発現

Outline of Final Research Achievements

We focused on differentiation of the lymphatic endothelial cells (LECs) and development of the lymphatic vessels in the craniofacial region of mouse embryos. ICR mouse embryos from E9.5-18.5 were used to collect the tissues in the craniofacial region. Gene expression profiling was performed using microarray, and was validated by qPCR and immunostaining. The gene expression analysis indicated that the high-level expression of Sox18, CoupTF2 and Prox1 showed at E11.5-12.5, E13.5 and E14.5, respectively. LECs differentiated from the venous endothelial cells (VECs) and sprouted in the cardinal veins of the truncal regions. Their LECs scattered, migrated away forward the craniofacial region, and arrived at the mandibular arches at E10.5. LECs aggregated, and the lymph sacs and lymphatic vessels were beginning to form after E12.5 and E14.5, respectively. We established the developmental processes of lymphatic vessels that originated from VECs of the cardinal veins in the craniofacial region.

Free Research Field

医歯薬学