2016 Fiscal Year Final Research Report
functional analysis of TMEM16E through its subsellular localozation.
| Project/Area Number |
26462813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Tobiume Kei 広島大学, 医歯薬保健学研究院(歯), 准教授 (40350037)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | TMEM16E / Ano5 / LGMD2L / GDD1 |
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| Outline of Final Research Achievements |
We developed a validated immunocytochemistry protocol for rabbit polyclonal TMEM16E
antibody in this study. Essentially, a special cell fixation reagent with a suitable detergent in a reaction buffer to erase non-specific reactions. By this sophisticated protocol, We identified TMEM16E/Caveolin3-carrying vesicle like structures in cultured myotube, which translocated just beneath plasma membrane in dysferlin-defect myotubes.
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| Free Research Field |
分子生物学
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