2016 Fiscal Year Final Research Report
A therapeutic approach to bone diseases by regulating osteoblast differentiation
| Project/Area Number |
26462817
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 骨代謝 / 骨芽細胞 / 細胞内シグナル伝達 / 細胞分化 / ＪＮＫ / オステオポンチン / オステオカルシン / 幹細胞 |
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| Outline of Final Research Achievements |
Osteopontin (OPN) is osteogenic marker protein which have regulatory effects on inflammatory diseases and bone metabolic disorders. Inhibition of JNK significantly inhibited osteogenic differentiation characterized by matrix mineralization and the gene expression of osteocalcin (OCN), whereas it increased OPN expression, indicating two types of osteogenic differentiation: OCN secretion type and OPN secretion type. The appearance of OPN type was decreased in DUSP16, a JNK specific phosphatase, knockout mouse. Gene expression analysis using DNA microarray revealed that OPN type osteoblasts specifically express secretory proteins and some surface markers. Moreover, inhibitor of differentiation 4 (Id4) suppressed the inhibitory effect of Hey1 on OPN promoter activity and regulated the induction of OPN type osteoblasts. Our findings suggested that JNK signaling regulates multifunctional differentiation of OPN and OCN type osteoblasts.
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| Free Research Field |
生化学
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