Establishment of physiological time measurement using saliva

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462830
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Onishi Yoshiaki 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 副研究部門長 (60233219)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 唾液 / 転写 / クロマチン / 生物時計

Outline of Final Research Achievements

Establishment of physiological time measurement using saliva is required for the advanced dental care based on biological rhythms. Then, we have found ARRB1 as a candidate of the marker for physiological time measurement in saliva from the results of the gene expression profile. ARRB1 protein showed the circadian profile even using various saliva samples, suggesting a usefulness of this marker protein. We also found that the buffer containing 4 M Urea is adequate for saliva storage to prevent proteolysis and that the rhythm in saliva should be corrected using Actin as an internal control.

Free Research Field

機能系基礎歯科学